Furin/PCSK3 His Tag protein, Mouse
SKU: 97186239198

Furin/PCSK3 His Tag protein, Mouse

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Description

Furin/PCSK3 His Tag protein, MouseProduct Specification Species Mouse Synonyms FUR,PCSK3,SPC1,PACE Accession P23188 Amino Acid Sequence Gln25 Glu714 with His Tag at the C Terminus Expression System HEK293 Molecular Weight 72 95kDa (Reducing) Purity 85% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer PBS, PH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Mouse
Synonyms FUR,PCSK3,SPC1,PACE
Accession P23188
Amino Acid Sequence

Gln25-Glu714 with His Tag at the C-Terminus

Expression System HEK293
Molecular Weight

72-95kDa (Reducing)

Purity >85% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose

Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· Please do not repeated freeze-thaw cycles.

Reference

1.Fitzgerald K. Furin Protease: From SARS CoV-2 to Anthrax, Diabetes, and Hypertension. Perm J. 2020;24:20.187.
2.Dahms SO, Hardes K, Becker GL, Steinmetzer T, Brandstetter H, Than ME. X-ray structures of human furin in complex with competitive inhibitors. ACS Chem Biol. 2014 May 16;9(5):1113-8.

Background

Furin, also known as Paired Basic Amino Acid Cleaving Enzyme (PACE), is a key member of the subtilisin-like proprotein convertase family. Functioning as a calcium-dependent serine protease, its structure includes an N-terminal signal peptide, a prodomain, a catalytic domain, a P-domain responsible for maintaining enzymatic activity and calcium/pH sensitivity, a transmembrane region, and a cytoplasmic tail. Primarily localized within the Golgi apparatus/endosomal system, it activates a wide range of proprotein substrates—such as growth factors, receptors, extracellular matrix proteins, and pathogen proteins (e.g., envelope proteins of HIV and influenza virus)—by specifically cleaving after the Arg-X-(Arg/Lys)-Arg sequence motif. It plays a critical role in embryogenesis, peripheral immune tolerance, and cellular homeostasis. Dysregulation of its function is closely linked to tumor progression, neurodegenerative diseases, and various pathogen infections, making it a significant therapeutic target in clinical applications.

Protocol

Assay protocol
Principle: Measured by its ability to cleave the fluorogenic peptide substrate, Pyr-Arg-Thr-Lys-Arg-AMC.
Materials
Assay buffer: 25 mM Tris, 1 mM CaCl2, pH 9.0
Furin/PCSK3 His Tag protein, Mouse (UA011277)
Substrate: Pyr-Arg-Thr-Lys-Arg-AMC (abs45133051)
96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
Plate Reader (PerkinElmer, excitation 380 nm and emission 460 nm)
Produce
Dilute Mouse Furin Protein to 2 µg/mL/4 µg/mL/8 µg/mL in Assay Buffer.
Dilute Substrate to 100 µM in Assay Buffer.
Load into a black well plate 50 µL of 2 µg/mL/4 µg/mL/8 µg/mL of Mouse Furin Protein, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 10 minutes.
Calculate specific activity:
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino-4-Methyl Coumarin
Conversion Factor (pmol/RFU)
Produce
Buffer: Assay buffer
Calibration standard: standard 7-amino-4-Methyl Coumarin (Enzo, Catalog # BML-KI107-0001).
Add 100 µL Dilute Calibration standard, Standard curve 1500/750/375/188/94/47/24/12/6/3 pmol per well or blank (Assay buffer) per well.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in endpoint mode.
Linear Regression of 7-amino-4-Methyl Coumarin (pmol) (y)—RFU-Blank(x).
Note
Storage Conditions: After reconstitution, aliquot and store at -80°C. Please do not repeated freeze-thaw cycles.

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SKU: 97186239198

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Tex
Waukegan, US
★★★★★ 4
Looks great by my bed!
Even my boyfriend keeps commenting how much he likes it. It's a usable piece of art! I will comment that the earring holes can be a little tricky since the leaves are turned all different ways. You have to be strategic with how you line up your long/dangle-y earrings with your studs so everybody fits and can be seen. A little tricky. But not a deal breaker - especially when it's so easy on the eye. The other slight negative is that this really isn't the best necklace holder. I would have thought, "No problem!" But it's a bit awkward. Especially if you actually intend to see all your earrings. You'll end up hanging most of your necklaces from the top most branch - but they slide down into the crevice to stay. Then you've got a lot of overlapping. And trickiness to retrieve the exact necklace you want with ease. However, this is still a fabulous earring tree. I can't say enough good things about it. Love the little basket for miscellaneous. Hair bands, lip gloss, etc.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 30, 2014
J
Verified Purchase
Joy Caldwell
Cuba, US
★★★★★ 5
Beautiful jewelry display
Color: Bronze-Tone
A few rough edges, but all in all well made and attractive. Holds lots of earrings and I can drape my necklaces on the tree. There's a nice bowl for bracelets and a nice tray that forms the base.
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Reviewed in the United States on April 27, 2024
K
Verified Purchase
K Carter
Natrona Heights, US
★★★★★ 5
Gorgeous
Color: Bronze-Tone
It really is pretty to look at. To be honest arranging the earring on the tree is a bit of a pain, because the leaves tilt in different directions, and you have to play with it a bit to get the earrings to hang the way you would like them, but once you figure it out, they look very nice. I just adore it because it looks so pretty. I use the basket on the side for rings and watches, and the little plate in the base for a few miscellaneous pieces like necklaces or bracelets for balance and because they look nice. You'll love it.
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Reviewed in the United States on November 16, 2024
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Verified Purchase
Jennifer Luo
Chelsea, US
★★★★★ 5
gorgeous, and worth the purchase.
Color: Bronze
I wasn't sure about purchasing the jewelry stand at first (I had my doubts), but those doubts quickly disappeared upon the arrival/unpackaging of the jewelry stand. SHIPPING/PACKAGING It was nicely packaged, and not bent or damaged when I recieved it. The stand was a little wobbly (due to careless measuring of the copper balls, the fault of the manufacturer) THE PRODUCT The pictures are not how it is. Remember that. I thought the tree would be more three dimensional, you know- like a real tree. It wasn't, though. It was like someone took a 2D copper tree and made it stand up. It's still pretty, though, and holds a lot of jewelry. PRICE-WISE If you're looking at the price and it says $45 or more, don't buy it. I waited a month for the price to go down (I'm just a cheapskate) and it did. It went from $45 or so, down to $25 or so. So, unless its life or death, be patient and buy it when the price is right. OVERALL? In general, I was pleased with the product. It wasn't my dream jewelry stand, but it was worth my money and the wait.
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Reviewed in the United States on January 3, 2013
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Verified Purchase
Shawnmariekerley
Boise, US
★★★★★ 5
Beautiful and sturdy
Color: Bronze, Color: Bronze
This piece is far superior than I expected. It is beautiful and sturdy. I don't have a lot of fancy jewelry. I just have several everyday pieces with a few unique pieces. This tree was perfect. It is attractive, like a unique piece of jewelry itself.
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Reviewed in the United States on February 6, 2024

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