Mouse IL-2 Kit(HICA)

Test Principle:

This kit utilizes a homogeneous immuno chemiluminescence assay (HICA) for the detection of cytokine concentrations. The operation is simple and requires no washing steps.

The detection system includes two types of microspheres: acceptor microspheres conjugated with antibody 1 targeting the protein of interest, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two microspheres into close proximity. When the distance between the donor and acceptor microspheres is less than 200 nm, singlet oxygen generated by light excitation can transfer to the acceptor microspheres, triggering chemiluminescence. Conversely, if the target protein is absent, the distance between the microspheres is too large, and no signal is produced.

By measuring the intensity of the chemiluminescent signal, quantitative analysis of the target protein can be achieved. This method offers advantages such as simplicity, rapid reaction, and high sensitivity.

Note: Microplates (384- or 96-well, white, shallow well)

Reagent R3 should be protected from light during use, and sample addition and incubation are recommended to be performed under green light (<100 LUX).

Recalibration is required for each test, with at least duplicate wells for each standard concentration point, and calculation should be performed using a four-parameter (weight 1/Y²) fitting method.

Temperature and time should be carefully controlled during incubation, and microplates should be covered with a film. A microplate reader with ALPHA function is recommended.

The diluent matrix for calibrators should be consistent with the test samples, and they should be used within 2 hours after reconstitution.

Components from different reagent kit lots must not be mixed.