Forskolin _ 51001ESForskolin is a natural diterpenoid product isolated from the Indian plant Coleus forskohlii that binds to the catalytic core of adenylyl cyclase (AC), which directly activates eukaryotic cell AC and increases cAMP levels in a variety of cells, and has been shown to activate all mammalian AC isoforms. In addition, Forskolin not only activates AC, but also interacts with certain other proteins, such as glucose transporters and ion channels. The

The amount of template added was optimized within the range of 1-10% of the reaction system

It contains His19-Val197

This can be detected by fluorescence microscopy or flow cytometry

The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/mL

the protein migrates to 45-48 kDa based on Tris-Bis PAGE result

Molecular cloning: Purify plasmids and recover enzyme digestion products

1% BSA to a concentration of 0

100 mM KCl and 0

In addition to inducing apoptosis

A:The analysis is conducted by observing the melting curve

1 week at 25°C

[Accession | F7E313]